Journal: International Journal of Clinical and Experimental Pathology

Article Title: Long noncoding RNA NORAD regulates lung cancer cell proliferation, apoptosis, migration, and invasion by the miR-30a-5p/ADAM19 axis

doi:

Figure Lengend Snippet: Expression level of NORAD in lung cancer tissues and cells. A. The expression level of NORAD in lung cancer tissues and adjacent normal samples (n=31) was tested by qRT-PCR. B. The abundance of NORAD was detected in lung cancer tissue, cells and HBE cells by qRT-PCR. *P<0.05.

Article Snippet: Small interfering RNAs (siRNAs) against NORAD (si-NORAD) or ADAM19 (si-ADAM19) and their negative control (si-NC), NORAD overexpression vector (NORAD) and vector, mimics or inhibitor of miR-30a-5p (miR-30a-5p or anti miR-30a-5p) and their negative control (miR-NC or anti-miR-NC) were bought from GenePharma (Shanghai, China).

Techniques: Expressing, Quantitative RT-PCR

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Long noncoding RNA NORAD regulates lung cancer cell proliferation, apoptosis, migration, and invasion by the miR-30a-5p/ADAM19 axis

doi:

Figure Lengend Snippet: Knockdown of NORAD suppressed progression in lung cancer. A549 and SCLC-21H cells were transfected with si-NC or si-NORAD. A. The level of NORAD was determined by qRT-PCR. B. MTT assay was used to estimate cell proliferation. C. Flow cytometry was performed to assess cell apoptosis. D and E. The number of migrated and invaded cells was determined by transwell assay. *P<0.05.

Article Snippet: Small interfering RNAs (siRNAs) against NORAD (si-NORAD) or ADAM19 (si-ADAM19) and their negative control (si-NC), NORAD overexpression vector (NORAD) and vector, mimics or inhibitor of miR-30a-5p (miR-30a-5p or anti miR-30a-5p) and their negative control (miR-NC or anti-miR-NC) were bought from GenePharma (Shanghai, China).

Techniques: Knockdown, Transfection, Quantitative RT-PCR, MTT Assay, Flow Cytometry, Transwell Assay

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Long noncoding RNA NORAD regulates lung cancer cell proliferation, apoptosis, migration, and invasion by the miR-30a-5p/ADAM19 axis

doi:

Figure Lengend Snippet: MiR-30a-5p is a target gene of NORAD. A. The binding sites between NORAD and miR-30a-5p were predicted by starBase. B. The luciferase activity was measured in A549 and SCLC-21H cells co-transfected with WT-NORAD or MUT-NORAD and miR-30a-5p or miR-NC. C. The level of miR-30a-5p was measured using qRT-PCR in lung cancer tissues and adjacent normal samples. D. The correlation between NORAD mRNA level and miR-30a-5p expression was analyzed in lung cancer tissues by Spearman’s correlation analysis. E. MiR-30a-5p level was detected by qRT-PCR in cells (HBE, A549 and SCLC-21H). F. The expression of NORAD was determined by qRT-PCR in A549 and SCLC-21H cells transfected with vector or NORAD. G. The level of miR-30a-5p was measured by qRT-PCR in A549 and SCLC-21H cells transfected with si-NC, si-NORAD, vector, or NORAD. *P<0.05.

Article Snippet: Small interfering RNAs (siRNAs) against NORAD (si-NORAD) or ADAM19 (si-ADAM19) and their negative control (si-NC), NORAD overexpression vector (NORAD) and vector, mimics or inhibitor of miR-30a-5p (miR-30a-5p or anti miR-30a-5p) and their negative control (miR-NC or anti-miR-NC) were bought from GenePharma (Shanghai, China).

Techniques: Binding Assay, Luciferase, Activity Assay, Transfection, Quantitative RT-PCR, Expressing, Plasmid Preparation

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Long noncoding RNA NORAD regulates lung cancer cell proliferation, apoptosis, migration, and invasion by the miR-30a-5p/ADAM19 axis

doi:

Figure Lengend Snippet: NORAD attenuated the inhibitory effect of miR-30a-5p on progression of lung cancer cells. A549 and SCLC-21H cells were transfected with miR-NC, miR-30a-5p, miR-30a-5p + vector, or miR-30a-5p + NORAD. A. MiR-30a-5p level was measured by qRT-PCR. B. MTT assay was used to estimate cell proliferation. C. Flow cytometry was performed to assess cell apoptosis. D and E. Transwell assay was employed to determine cell migration and invasion. *P<0.05.

Article Snippet: Small interfering RNAs (siRNAs) against NORAD (si-NORAD) or ADAM19 (si-ADAM19) and their negative control (si-NC), NORAD overexpression vector (NORAD) and vector, mimics or inhibitor of miR-30a-5p (miR-30a-5p or anti miR-30a-5p) and their negative control (miR-NC or anti-miR-NC) were bought from GenePharma (Shanghai, China).

Techniques: Transfection, Plasmid Preparation, Quantitative RT-PCR, MTT Assay, Flow Cytometry, Transwell Assay, Migration

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Long noncoding RNA NORAD regulates lung cancer cell proliferation, apoptosis, migration, and invasion by the miR-30a-5p/ADAM19 axis

doi:

Figure Lengend Snippet: ADAM19 is regulated by NORAD and miR-30a-5p. A549 and SCLC-21H cells were transfected with miR-NC, miR-30a-5p, miR-30a-5p + vector, or miR-30a-5p + NORAD. A and B. The mRNA and protein expression levels of ADAM19 were detected using qRT-PCR and western blot, respectively. *P<0.05.

Article Snippet: Small interfering RNAs (siRNAs) against NORAD (si-NORAD) or ADAM19 (si-ADAM19) and their negative control (si-NC), NORAD overexpression vector (NORAD) and vector, mimics or inhibitor of miR-30a-5p (miR-30a-5p or anti miR-30a-5p) and their negative control (miR-NC or anti-miR-NC) were bought from GenePharma (Shanghai, China).

Techniques: Transfection, Plasmid Preparation, Expressing, Quantitative RT-PCR, Western Blot